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To determine whether the constructed plasmid was efficient
works well, we first transfected the plasmid with liposomes into cultured SH-SY5Y cells for 24 hours and analyzed the expression of corresponding proteins byusedwestern blot analysis using anwithantibody against EGFPto check the expression of corresponding proteins.
We screened aA pool of
ArabidospisArabidopsis lines transformed with the activation vector was screened for short hypocotyl mutants under dim far-red light.Consequently we isolated, and three mutant lines designated chibi1-3 (chi) were isolated.
Rats were sacrificed
killedat different time points followingofreperfusion. Left hemisphericFfrontal and parietal lobe cortex tissuesonthe left hemisphere(6 to 11 mm fromtothe tip of olfactory bulb) were separated and saved in liqguid nitrogen.
Rats
whichwere supplemented with further injections as necessary during experiments to sustain a level of acceptable anesthesia. Body temperature was maintained at 36-37C using a heating pad placed underneath the rat.. Each ratsThe heads waswaserefixed horizontally in a stereotaxic instrument (Narishige, Japan). The skull was exposed,and a small burr hole was made at the appropriate coordinatesdrilledto enable vertical penetration by the stimulating and recording electrodes. All electrodes were zeroedon theat bregma,and all coordinates were calculated from this point.
WeThese experiments demonstrated a distinctive localization of OMPFC neurons, which revealed excitatory responses to BLA stimulation, althoughPrez-Jaranay and Vivesa previous study did not demonstrate this apparent distribution of the excitatory responses (Prez-Jaranay and Vives, 1991).
W
Here we suggestthata comprehensive molecular genetic study involving a large number of NF2is encouragedto build up our Chinese gene mutation database not only for the benefitforoftheclinical practice, but also for genetic counseling purposess.
It is
highreasonable tobelievesuggest that these NATs are critical for the life of humans,mousemice and rats,justbecause theyare stillremain in the transcriptome after tens of million years of evolution.
A total of 570 NATs were selected,
tripled ofthree times the numberlehnerreportedearlierin a previous study (8),andwith most of themare thebeing reported for the first timetobe reported.
It was noted that the onset of SWS1 expression in our study was earlier than
thethatonereported previously [26] where the expression initiatedsbetween 55 and 60 hpfandwith about half of the embryos showing expression at 60 hpf butare stillremaining in stage 1.In theirThe aforementioned study differed from ours in that only fish that had achieved certain developmental characteristics at certain time points were examined for opsin expression to minimize the individual variation [26], while we did not perform any such selection. The timedifferencediscrepancy between the two studies is most likely to be due to this differenceoriginate fromit.
By constructing DB2GO, the entries in the BioDW member databases
of BioDWare linked organically to the terms of Gene Ontology Database.organically. In the DB2GO table,Iit is quite easy to find entries in the DB2GO table from different databases as theywhichall areallannotated by the same GO term,from different databases.
[Author: You should mention the 3 treatment groups here since this is really part of the study design (like you did in the \’Methods\’ section but in less detail).]